1、下載安裝MrBayes
http://nbisweden.github.io/MrBayes/
git clone --depth=1 https://github.com/NBISweden/MrBayes.git
cd?MrBayes
./configure --prefix=/work/workspace/username/program/MrBayes
ln -s? /work/workspace/username/program/MrBayes/bin/mb?/work/workspace/username/bin
2独令、fasta 轉(zhuǎn)?nexus
https://www.ebi.ac.uk/Tools/msa/clustalo/
將sequence.nexus上傳到文件夾Dir4MrBayes
3、設(shè)置突變模型invgamma达吞，1M次Bayes建樹
mb
MrBayes > Execute? sequence.nexus;
MrBayes > prset statefreqpr=fixed(empirical); lset rates=invgamma;
MrBayes > mcmcp ngen=1000000 nchains=4 printfreq=100 samplefreq=100 burnin=200;
MrBayes >?mcmc;
MrBayes >?sump;
MrBayes >?sumt;
MrBayes >?quit
4宁赤、展示最后結(jié)果FigTree
下載FigTree?https://github.com/rambaut/figtree/releases
運(yùn)行FigTree v1.4.4
載入sequence.nexus.con.tre
Appearance -> 設(shè)置分支格式, Line Weight: 2
Tip Labels -> 設(shè)置字體格式鉴逞，F(xiàn)ont: Arial, size: 14
Branch Labels -> 設(shè)置分支標(biāo)簽格式枝冀，Display: prob(percent)? Font: Arial, size: 14,?
Scale Axis -> 設(shè)置圖例標(biāo)尺格式，F(xiàn)ont size: 14,?Line Weight: 2
File -> Export PDF
Tips: 修改環(huán)形展示方式
Layout: Polar tree layout
