創(chuàng)建錄制宏:打開一個圖片 --- Plugins --- Macros --- Record --- name it
格式轉(zhuǎn)化:Image --- Type --- 8-bit(為灰度圖片)
增強對比度:Process --- Enhance contrast (選“normalize”+"0.3%")
平滑處理:Process --- Smooth
尋找邊緣:Process --- Find Edges
調(diào)節(jié)閾值:Image --- Adjust --- Threshold(分別為“0” “20”)--- Apply
魔棒工具:使用魔棒工具選擇劃痕區(qū)域
劃痕面積:Analyze --- Measure
保存錄制宏:Create --- file --- save --- 圖片所在的文件夾
傷口愈合百分比(Wound healing percentage):(0h面積-xh面積)/ 0h面積
ImageJ處理劃面積:https://www.bilibili.com/video/BV13b41177ny/