Workflow of NGS 二代測序流程
- Sample fragmentation
- Library preparation
- Clonal Amplification
- Sequencing reaction
- Data analysis
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Principle of SMRT
- Millions of zero-mode waveguides (ZMWs), embedded with only one set of enzymes and DNA template that can be detected during the whole process.
- During the reaction, the enzyme will incorporate the nucleotide into the complementary strand and cleave off the fluorescent dye previously linked with nucleotide.
- The camera inside the machine will capture signal in a movie format in real-time observation.
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整理自MOOC
