LEARNING OUTCOMES
Describe how water potential influences how water is transported in plants

Plants are phenomenal hydraulic engineers. Using only the basic laws of physics and the simple manipulation of potential energy, plants can move water to the top of a 116-meter-tall tree (Figure 1a). Plants can also use hydraulics to generate enough force to split rocks and buckle sidewalks (Figure 1b). Plants achieve this because of water potential.

Figure 1. With heights nearing 116 meters, (a) coastal redwoods (Sequoia sempervirens) are the tallest trees in the world. Plant roots can easily generate enough force to (b) buckle and break concrete sidewalks, much to the dismay of homeowners and city maintenance departments. (credit a: modification of work by Bernt Rostad; credit b: modification of work by Pedestrians Educating Drivers on Safety, Inc.)

Water potential is a measure of the potential energy in water. Plant physiologists are not interested in the energy in any one particular aqueous system, but are very interested in water movement between two systems. In practical terms, therefore, water potential is the difference in potential energy between a given water sample and pure water (at atmospheric pressure and ambient temperature). Water potential is denoted by the Greek letter ψ (psi) and is expressed in units of pressure (pressure is a form of energy) called megapascals (MPa). The potential of pure water (Ψ_w^{pure H2O}) is, by convenience of definition, designated a value of zero (even though pure water contains plenty of potential energy, that energy is ignored). Water potential values for the water in a plant root, stem, or leaf are therefore expressed relative to Ψ_w^{pure H2O}.

The water potential in plant solutions is influenced by solute concentration, pressure, gravity, and factors called matrix effects. Water potential can be broken down into its individual components using the following equation:

Ψ_system = Ψ_total = Ψ_s + Ψ_p + Ψ_g + Ψ_m

where Ψ_s, Ψ_p, Ψ_g, and Ψ_m refer to the solute, pressure, gravity, and matric potentials, respectively. “System” can refer to the water potential of the soil water (Ψ^soil), root water (Ψ^root), stem water (Ψ^stem), leaf water (Ψ^leaf) or the water in the atmosphere (Ψ^atmosphere): whichever aqueous system is under consideration. As the individual components change, they raise or lower the total water potential of a system. When this happens, water moves to equilibrate, moving from the system or compartment with a higher water potential to the system or compartment with a lower water potential. This brings the difference in water potential between the two systems (ΔΨ) back to zero (ΔΨ = 0). Therefore, for water to move through the plant from the soil to the air (a process called transpiration),Ψ^soil must be > Ψ^root > Ψ^stem > Ψ^leaf > Ψ^atmosphere.

Water only moves in response to ΔΨ, not in response to the individual components. However, because the individual components influence the total Ψ_system, by manipulating the individual components (especially Ψ_s), a plant can control water movement.

Solute Potential

Solute potential (Ψ_s), also called osmotic potential, is negative in a plant cell and zero in distilled water. Typical values for cell cytoplasm are –0.5 to –1.0 MPa. Solutes reduce water potential (resulting in a negative Ψ_w) by consuming some of the potential energy available in the water. Solute molecules can dissolve in water because water molecules can bind to them via hydrogen bonds; a hydrophobic molecule like oil, which cannot bind to water, cannot go into solution. The energy in the hydrogen bonds between solute molecules and water is no longer available to do work in the system because it is tied up in the bond. In other words, the amount of available potential energy is reduced when solutes are added to an aqueous system. Thus, Ψ_s decreases with increasing solute concentration. Because Ψ_s is one of the four components of Ψ_system or Ψ_total, a decrease in Ψ_s will cause a decrease in Ψ_total. The internal water potential of a plant cell is more negative than pure water because of the cytoplasm’s high solute content (Figure 2). Because of this difference in water potential water will move from the soil into a plant’s root cells via the process of osmosis. This is why solute potential is sometimes called osmotic potential.

Plant cells can metabolically manipulate Ψ_s (and by extension,Ψ_total) by adding or removing solute molecules. Therefore, plants have control over Ψ_total via their ability to exert metabolic control over Ψ_s.

Figure 2. A semipermeable membrane between two aqueous systems

In Figure 2, water will move from a region of higher to lower water potential until equilibrium is reached. Solutes (Ψ_s), pressure (Ψ_p), and gravity (Ψ_g) influence total water potential for each side of the tube (Ψ_total ^{right or left}), and therefore, the difference between Ψ_total on each side (ΔΨ). (Ψ_m, the potential due to interaction of water with solid substrates, is ignored in this example because glass is not especially hydrophilic). Water moves in response to the difference in water potential between two systems (the left and right sides of the tube).

Pressure Potential

Pressure potential (Ψ_p), also called turgor potential, may be positive or negative (Figure 2). Because pressure is an expression of energy, the higher the pressure, the more potential energy in a system, and vice versa. Therefore, a positive Ψ_p (compression) increases Ψ_total, and a negative Ψ_p (tension) decreases Ψ_total. Positive pressure inside cells is contained by the cell wall, producing turgor pressure. Pressure potentials are typically around 0.6–0.8 MPa, but can reach as high as 1.5 MPa in a well-watered plant. A Ψ_p of 1.5 MPa equates to 210 pounds per square inch (1.5 MPa x 140 lb in^-2 MPa^-1 = 210 lb/in^-2). As a comparison, most automobile tires are kept at a pressure of 30–34 psi. An example of the effect of turgor pressure is the wilting of leaves and their restoration after the plant has been watered (Figure 3). Water is lost from the leaves via transpiration (approaching Ψ_p = 0 MPa at the wilting point) and restored by uptake via the roots.

A plant can manipulate Ψ_p via its ability to manipulate Ψs and by the process of osmosis. If a plant cell increases the cytoplasmic solute concentration, Ψ_s will decline, Ψ_total will decline, the ΔΨ between the cell and the surrounding tissue will decline, water will move into the cell by osmosis, and Ψ_p will increase. Ψ_p is also under indirect plant control via the opening and closing of stomata. Stomatal openings allow water to evaporate from the leaf, reducing Ψ_p and Ψ_total of the leaf and increasing ΔΨ between the water in the leaf and the petiole, thereby allowing water to flow from the petiole into the leaf.

Figure 3. When (a) total water potential (Ψtotal) is lower outside the cells than inside, water moves out of the cells and the plant wilts. When (b) the total water potential is higher outside the plant cells than inside, water moves into the cells, resulting in turgor pressure (Ψp) and keeping the plant erect. (credit: modification of work by Victor M. Vicente Selvas)

Gravity Potential

Gravity potential (Ψ_g) is always negative to zero in a plant with no height. It always removes or consumes potential energy from the system. The force of gravity pulls water downwards to the soil, reducing the total amount of potential energy in the water in the plant (Ψ_total). The taller the plant, the taller the water column, and the more influential Ψ_g becomes. On a cellular scale and in short plants, this effect is negligible and easily ignored. However, over the height of a tall tree like a giant coastal redwood, the gravitational pull of –0.1 MPa m^-1 is equivalent to an extra 1 MPa of resistance that must be overcome for water to reach the leaves of the tallest trees. Plants are unable to manipulate Ψ_g.

Matric Potential

Matric potential (Ψ_m) is always negative to zero. In a dry system, it can be as low as –2 MPa in a dry seed, and it is zero in a water-saturated system. The binding of water to a matrix always removes or consumes potential energy from the system. Ψ_m is similar to solute potential because it involves tying up the energy in an aqueous system by forming hydrogen bonds between the water and some other component. However, in solute potential, the other components are soluble, hydrophilic solute molecules, whereas in Ψ_m, the other components are insoluble, hydrophilic molecules of the plant cell wall. Every plant cell has a cellulosic cell wall and the cellulose in the cell walls is hydrophilic, producing a matrix for adhesion of water: hence the name matric potential. Ψ_m is very large (negative) in dry tissues such as seeds or drought-affected soils. However, it quickly goes to zero as the seed takes up water or the soil hydrates. Ψ_m cannot be manipulated by the plant and is typically ignored in well-watered roots, stems, and leaves.

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