?一变擒、α多樣性指數(shù)分析內(nèi)容及意義

a) 群落生態(tài)學中研究微生物多樣性寝志，通過單樣品的多樣性分析（α[Alpha]多樣性）可以反映微生物群落的豐度和多樣性娇斑，包括一系列統(tǒng)計學分析指數(shù)估計環(huán)境群落的物種豐度和多樣性材部。?

b) α多樣性指分析主要包括：

①菌群豐度（Community richness）指數(shù)計算：Chao、Ace指數(shù)乐导；

②菌群多樣性（Community diversity）指數(shù)計算：Shannon、Simpson指數(shù)物臂。

? ? ——獲得指數(shù)計算匯總表格。

c) α多樣性可視化分析圖（來源：指數(shù)分析匯總表）：

稀釋性曲線（Rarefraction Curve）棵磷；Shannon-Wiener曲線；Rank-Abundance曲線仪媒；Specaccum曲線

二、α多樣性分析在科技論文中的描述

a) 稀釋性曲線和Shannon-Wiener曲線結(jié)合使用：

例如1：Shannon and Rarefaction analysis showed that, although new phylotypes can be obtained by additional sequencing, most of the gut microbial diversity in each sample was captured with the current sequencing depth. After rarefying the sequencing depth among all the samples using bootstrap (XX,XXX reads per sample), Shannon diversity index and rarefaction OTU estimates were calculated. There was no significant difference in the richness (as indicated by rarefaction OTU estimates) and diversity between Group A and Group B in this study.

如有顯著差異：while Group B significantly reduced both the richness and diversity of the microbiota(P<0.05 OR Show in fig.)留凭。

? 例 如2：The richness of oral bacterial communities in three oral habitats of periodontitis patients and the control cohort were estimated by rarefaction and Chao 1, and diversity was estimated by Shannon diversity index and Simpson diversity. The shape of the rarefaction curves indicated new phylotypes would be expected with additional sequencing. However, the Shannon diversity index curves of all samples reached plateaus with the current sequencing, suggesting that most diversity had already been captured.

b) Rank-abundance曲線：

例如1：Rank-abundance curves reflect the fact that a few dominant phylotypes comprise the major proportion of the A communities whereas a more even community is seen in XXX.

例如2：Bacterial communities were also similar in their structure, exhibiting comparable trends for both rank frequency and abundance across the soils sampled.

c) 物種累積曲線：

例如：According to the sample number and species OTUs, we calculated the species accumulation curve of all participants. In this study, the curve had reached a plateau, and the species had no more obvious increase as the sample number increased, which indicated that the sample volume in our study was relatively large enough to reflect the species richness.

d) α多樣性指數(shù)比較：

? 例 如：We compared the α-diversity of microbiota between XX and XX samples using the chao1, ACE, observed species, Shannon and Simpson index, and found that all five indexes showed significant difference (P = XXX, XXX, XXX, XXX, and XXX, respectively). The XXX samples had a significantly higher α-diversity.

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