Formative pluripotency: the executive phase in a developmental continuum - PubMed (nih.gov)
摘要
The regulative capability of single cells to give rise to all primary embryonic lineages is termed pluripotency. Observations of fluctuating gene expression and phenotypic heterogeneity in vitro have fostered a conception of pluripotency as an intrinsically metastable and precarious state. However, in the embryo and in defined culture environments the properties of pluripotent cells change in an orderly sequence. Two phases of pluripotency, called na?ve and primed, have previously been described. In this Hypothesis article, a third phase, called formative pluripotency, is proposed to exist as part of a developmental continuum between the na?ve and primed phases. The formative phase is hypothesised to be enabling for the execution of pluripotency, entailing remodelling of transcriptional, epigenetic, signalling and metabolic networks to constitute multi-lineage competence and responsiveness to specification cues
單細(xì)胞產(chǎn)生所有初級(jí)胚胎譜系的調(diào)節(jié)能力稱(chēng)為多能性究流。對(duì)波動(dòng)的基因表達(dá)和體外表型異質(zhì)性的觀察產(chǎn)生了多能性作為一種內(nèi)在亞穩(wěn)態(tài)和不穩(wěn)定狀態(tài)的概念。然而盏袄，在胚胎和特定的培養(yǎng)環(huán)境中仪媒，多能細(xì)胞的特性會(huì)按順序發(fā)生變化戚啥。之前已經(jīng)描述了多能性的兩個(gè)階段祠斧，稱(chēng)為naive和primed吞琐。在這篇文章中砂轻，第三階段奔誓，稱(chēng)為formative多能性，被提議作為naive階段和primed階段之間的發(fā)展連續(xù)體的一部分而存在舔清。formative階段被認(rèn)為能夠執(zhí)行多能性丝里，重塑轉(zhuǎn)錄、表觀遺傳体谒、信號(hào)傳導(dǎo)和代謝網(wǎng)絡(luò)杯聚，以構(gòu)成多譜系能力和對(duì)分化線索的反應(yīng)。
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