1.詞匯：
Infiltrates n. 浸潤物（infiltrate的復(fù)數(shù)）v. 浸潤（infiltrate的第三人稱單數(shù)）
Malignancy n. 惡性（腫瘤等）加勤；惡意
Metastasis n. 轉(zhuǎn)移婆硬；新陳代謝瘩燥；[地質(zhì)] 同質(zhì)蛻變
Gastrointestinal adj. 胃腸的
malignant melanoma n.惡性黑色素瘤
Urgent adj. 緊急的暂雹；急迫的
Elucidation n. 說明；闡明
Hyaluronan n. 透明質(zhì)酸
Motility n. 運(yùn)動(dòng)性仗岖；能動(dòng)性逃延；自動(dòng)力
Deconvolution n. [地質(zhì)] 反褶積览妖，[計(jì)] 去卷積
Adjacent adj. 鄰近的轧拄，毗連的
Homologous adj. 相應(yīng)的；[生物] 同源的讽膏；類似的檩电；一致的
2.語句：
However, the underlying functions and mechanisms of LAYN in tumor progression and tumor immunology is still unclear.
The findings in this report shed light on the important role of LAYN in colorectal and gastric cancers as well as provide a potential relationship and an underlying mechanism between LAYN and tumor-immune interactions.
GEPIA (29) is an interactive web that includes 9,736 tumors and 8,587 normal samples from TCGA and the GTEx projects, which analyse the RNA sequencing expression.
Here the N category refers to lymph node involvement; N0 indicates no regional lymph node metastasis, and N1–N3 indicate regional lymph node metastasis
Tumor-infiltrating lymphocytes are an independent predictor of sentinel lymph node status and survival in cancers
INTRODUCTION:
胃腸癌是全世界男女共患的最常見的惡性腫瘤，并且其轉(zhuǎn)移常導(dǎo)致不良預(yù)后府树。免疫相關(guān)機(jī)制在胃腸癌中至關(guān)重要俐末，免疫療法被認(rèn)為是治療GI有前景的方法。免疫療法奄侠，比如CTLA4,PD-1,PD-L1抑制物卓箫，在惡性黑色素瘤和非小細(xì)胞肺癌中，展現(xiàn)出可靠的抗腫瘤效果垄潮。但是烹卒，目前的免疫療法存在缺陷，anti-CILA4在轉(zhuǎn)移性的結(jié)直腸癌和胃癌臨床治療效果并不理想弯洗，同樣anti-PD-1和anti-PD-L1在進(jìn)展性的胃癌和結(jié)直腸癌中治療效果大打折扣旅急。另外，一些研究表明牡整，腫瘤浸潤淋巴細(xì)胞（tumor-infiltrating lymphocytes）藐吮，比如tumor-associated macrophages (TAMs)和tumor-infiltrating neutrophils (TINs)，它們能夠影響預(yù)后和免疫療法以及化療的效果逃贝。因此谣辞，急需闡明腫瘤免疫互作的免疫表型，確定結(jié)直腸癌和胃癌的新的免疫靶標(biāo)分子沐扳！
Layilin（LAYN）在1998年首次被報(bào)道泥从，它是一種位于11號染色體的編碼基因，作為一種55-kDa的跨膜蛋白迫皱，在多種細(xì)胞和器官中表達(dá)歉闰。LAYN是一種透明質(zhì)酸的表面受體辖众，參與細(xì)胞間黏附、運(yùn)動(dòng)和轉(zhuǎn)移調(diào)控和敬；LAYN調(diào)控腎小管上皮細(xì)胞的TNF-α-EMT凹炸，在炎性腸病中參與HA35誘導(dǎo)的小腸上皮間緊密連接。LYAN首次被證明在肺腺癌細(xì)胞系A(chǔ)549中與侵襲和淋巴結(jié)轉(zhuǎn)移有關(guān)昼弟。
LAYN表達(dá)是CRC和NSCLC患者樣本的調(diào)性T淋巴細(xì)胞(Treg)浸潤的特異性特征啤它。此外，在CRC和NSCLC患者中舱痘，LAYN的高表達(dá)與不良預(yù)后相關(guān)(18)变骡。先前的研究(19)進(jìn)一步發(fā)現(xiàn)LAYN是參與肝癌腫瘤浸潤淋巴細(xì)胞的關(guān)鍵基因。T細(xì)胞單細(xì)胞RNA測序證實(shí)芭逝，體外活化的CD8+ T和Treg細(xì)胞中LAYN上調(diào)塌碌，抑制CD8+ T細(xì)胞功能。
However, the underlying functions and mechanisms of LAYN in tumor progression and tumor immunology is still unclear.
MATERIALS AND METHODS：
Oncomine數(shù)據(jù)庫(https://www.oncomine.org/resource/login.html)
Kaplan-Meier Plotter(http://kmplot.com/analysis/
PrognoScan 數(shù)據(jù)庫(http://www.abren.net/PrognoScan/)
TIMER數(shù)據(jù)庫(https://cistrome.shinyapps.io/timer/)
GEPIA數(shù)據(jù)庫(http://gepia.cancer-pku.cn/index.html)
RESULTS：
1.用Oncomine數(shù)據(jù)分析了不同癌癥中LAYN的表達(dá)水平旬盯，高表達(dá)的有breast, colorectal, gastric,kidney, pancreatic cancers, and lymphoma tumors台妆；低表達(dá)的有bladder, breast, colorectal, head and neck,lung, ovarian and prostate cancers。另外胖翰，分析了TCGA來自多個(gè)惡性腫瘤的RNA-seq數(shù)據(jù)接剩，低表達(dá)有BLCA (bladder urothelial carcinoma), BRCA (breast invasive carcinoma), COAD (colon adenocarcinoma), KICH (kidney chromophobe), LUAD (lung adenocarcinoma), PRAD (prostate adenocarcinoma), READ (rectum adenocarcinoma), STAD (stomach adenocarcinoma), THCA (thyroid carcinoma), UCEC (uterine corpus endometrial carcinoma)；高表達(dá)有CHOL (cholangiocarcinoma), HNSC (head and neck cancer), KIRC (kidney renal clear cell carcinoma), LIHC (liver hepatocellular carcinoma)萨咳。
2.作者首先用PrognoScan數(shù)據(jù)庫分析了不同癌癥中LAYN表達(dá)與預(yù)后的相關(guān)性懊缺，發(fā)現(xiàn)LAYN與結(jié)直腸癌、乳腺癌培他、眼癌鹃两、卵巢癌預(yù)后相關(guān)，其中在結(jié)直腸癌中靶壮，高表達(dá)LAYN與預(yù)后不良相關(guān)怔毛。然后，作者用Kaplan-Meier plotter數(shù)據(jù)庫也作了預(yù)后分析腾降，發(fā)現(xiàn)LAYN與卵巢癌和胃癌預(yù)后相關(guān)拣度；除此之外，作者還用GEPIA數(shù)據(jù)庫分析了來自TCGA的33種癌癥的數(shù)據(jù)螃壤，發(fā)現(xiàn)COAD, OV,HNSC, MESO ,GBM ,KIRP ,THCA,SKCM 預(yù)后與LAYN表達(dá)相關(guān)抗果。
3.用Kaplan-Meier plotter數(shù)據(jù)庫分析了胃癌樣本不同臨床特征亞組下LAYN與預(yù)后的關(guān)系，發(fā)現(xiàn)LAYN表達(dá)主要影響有淋巴結(jié)轉(zhuǎn)移的胃癌患者預(yù)后奸晴。
4.作者用TIMER數(shù)據(jù)庫分析了不同癌癥中LYAN表達(dá)與免疫浸潤之間的關(guān)系冤馏，由于TIMER和GEPIA數(shù)據(jù)都是來自于TCGA，所以作者挑選了在TIMER中與腫瘤純度負(fù)相關(guān)的同時(shí)在GEPIA中與預(yù)后顯著相關(guān)的寄啼，最后選出了結(jié)腸癌和胃癌逮光。
5.作者用分析了LAYN和免疫標(biāo)志基因集的相關(guān)性代箭，同樣發(fā)現(xiàn)LAYN在結(jié)腸癌和胃癌的免疫逃脫中起到至關(guān)重要的作用。
綜上所述涕刚，LAYN表達(dá)升高與多種癌癥特別是結(jié)腸癌和胃癌的CD8+ T細(xì)胞嗡综、CD4+ T細(xì)胞、巨噬細(xì)胞杜漠、中性粒細(xì)胞和DCs的不良預(yù)后及免疫浸潤水平升高相關(guān)极景。此外，在胃癌和結(jié)腸癌中驾茴，LAYN表達(dá)可能參與調(diào)控腫瘤相關(guān)巨噬細(xì)胞(TAMs)盼樟、DCs、T細(xì)胞衰竭和Tregs锈至。作為胃癌和結(jié)腸癌患者預(yù)后的生物標(biāo)志物晨缴。因此，LAYN可能在免疫細(xì)胞中發(fā)揮重要作用
