sliva rRNA數(shù)據(jù)庫(http://www.arb-silva.de/)用來檢查和比對RNA序列,既可以針對16S/18S,SSU,也可以針對23S/28S, LSU仔役,包括了Bacteria, Archaea and Eukarya察皇。同時也是ARB的官方指定數(shù)據(jù)庫宁炫。
LSU: Large subunit (23S/28Sribosomal RNAs) 大亞基
細(xì)菌暴备、古菌16S rRNA
SSU: Small subunit (16S/18Sribosomal RNAs) 小亞基
真菌28S rRNA
僅僅是下載fasta文件
下載地址:http://www.arb-silva.de/no_cache/download/archive/release_119/Exports/
根據(jù)下載的需求围小,選擇針對23S/28Sribosomal RNAs的LSU或者是針對16S/18Sribosomal RNAs的SSU宪迟;然后選擇是否去冗余的酣衷,我選擇去,即Nr99;然后選擇是否trunc次泽,即是否對名字縮寫穿仪;選擇是否全長比對結(jié)果;
*_tax_silva.fasta.gz
Multi FASTA files of the SSU/LSU databases including the SILVAtaxonomy for
Bacteria, Archaea and Eukaryotes in the header.
REMARK: The sequences in the files are NOT truncated to theeffective LSU or
SSU genes. They contain the full entries as they have been depositedin the
public repositories (ENA/GenBank/DDBJ).
Fasta header:
accession_number.start_position.stop_position taxonomic pathorganism name
*_tax_silva_full_align_trunc.fasta.gz
Multi FASTA files of the SSU/LSU databases including the SILVAtaxonomy for
Bacteria, Archaea and Eukaryotes in the header (including the FULLalignment).
REMARK: Sequences in these files haven been truncated. This meansthat all
nucleotides that have not been aligned were removed from thesequence.
*_tax_silva_trunc.fasta.gz
Multi FASTA files of the SSU/LSU database including the SILVAtaxonomy for
Bacteria, Archaea and Eukaryotes in the header.
REMARK: Sequences in these files haven been truncated. This meansthat all
nucleotides that have not been aligned were removed from thesequence.
生成使用與Mothur的silva數(shù)據(jù)庫:http://blog.mothur.org/2014/08/08/SILVA-v119-reference-files/