背景：
Z. mays immune signalling peptide 1 (Zip1):17 amino acid peptide [+EGESELKLATQGASVRR–]
corn smut?[植保] 玉米黑粉病
玉米黑粉病由玉米黑粉菌（Ustilago maydis）（活體寄生biotrophic
fungus）所引起的一種局部侵染性病害。孢子堆的小大氧吐、形狀不定，多呈瘤狀僚楞，長或直徑3-15cm譬淳，初期外面有一層白色膜，往往由寄生組織形成，有時還帶黃綠色或紫紅色彩宛畦，后漸變灰白至灰色，破裂后散出大量黑色粉末揍移，即冬孢子次和。
E-6427, a specific PLCP inhibitor
結(jié)果：
In vitro studies demonstrate that PLCPs arerequired to release bioactive Zip1 from its propeptide precursor. Conversely,Zip1 treatment strongly elicits SA accumulation in leaves. Zip1 promotes theinfection of the necrotrophic fungus Botrytis cinerea,while it reducesvirulence of the biotrophic fungus Ustilago maydis.
日積月累：
aerial parts? 地上部分
activity-based protein profiling (ABPP)
DCG-04, a specific probe for the detectionof active PLCPs
damage-associated molecular patterns(DAMPs)
Papain-like cysteine proteases (PLCPs)
Papain [p?'pe??n; p?'pa??n]/ [p?'pe?n]?n. [生化] 木瓜蛋白酶
fungi[?f??ɡa?; ?f?nd?a?; ?f?nd??]
n. 真菌；菌類那伐；蘑菇（fungus的復數(shù)）
fungus?['f??g?s]? n.真菌踏施，霉菌石蔗；菌類
propeptide?前肽
cuticle?['kju?t?k(?)l]? n.角質(zhì)層；表皮畅形；護膜
pathogenesis n.發(fā)病機理
apoplastic??n.非原質(zhì)體的
fine-tuned?adj.有調(diào)整的养距，微調(diào)的；有提高的 v. 調(diào)整日熬；使有規(guī)則棍厌；對進行微調(diào)
ectopically?異位表達
cystatin?半胱氨酸蛋白酶抑制劑
reciprocal英[r?'s?pr?k(?)l]? adj.互惠的；相互的竖席；倒數(shù)的耘纱，彼此相反的n. [數(shù)] 倒數(shù)；互相起作用的事物
strikingly?['stra?k??li]?adv.顯著地毕荐，突出地束析，引人注目地
1. Localized control of cell death iscrucial for the resistance of plants to pathogens.
2. Papain-like cysteine proteases (PLCPs)regulate plant defence to drive cell death and protection against biotrophicpathogens.
3. In maize (Zea mays), PLCPsare crucial in the orchestration ofsalicylic acid(SA)-dependent defence signalling.
4. Plants face a wide range of bioticthreats including viruses, bacteria, insects and fungi.
5. Further, the regulation of plantimmunity also commonlyinvolves the fine-tunedinterplay of
phytohormones such as SA, jasmonic acid(JA) and ethylene (ET).
6. Among defence-related phytohormones, SA is a key player that orchestrates responses to bothbiotic and abiotic stresses andextensive studies have
detailedthe role of SA in innate immune signaling.
7. SA signalling promotes efficient defenceactivation against biotrophic pathogens, whereas necrotrophic pathogens aresensitive to JA/ET-dependent defence signalling.
8. Together, these experiments demonstratethat Zip1 activity closely mirrors SA signalling and predictably promotesdisease caused by necrotrophic and biotrophic fungi.
文章還有一些有意思的實驗技術(shù)和方法，整理好后在與大家分享东跪。
