hisat2 align
begining to analysis raw data, you should be clean the fastq data. Here we use the hisat2 software to do this project.
#usr/bin/perl
use strict;
open IN,"sqf.sh";
my %fg;
my @gg;
while (<IN>) {
print $_;
}
#usr/bin/perl
use strict;
open I,"Homo_sapiens.GRCh38.81.gtf";
open IO,">Homo_sapiens.gtf";
while (<I>) {
chomp;
my @ss = split/\t/;
next if($ss[0] =~ m/CHR/);
my ($a)= (split/ /,$ss[-1])[1];
my $al = join"\t",@ss[0..(@ss-2)];
$a =~ s/\"http://g;
$a =~ s/\;//g;
print IO $al."\t".$a."\n";
# body...
}
<img src="C:\Users\Lenovo\Desktop\iI\微信圖片_20200814122753.jpg" alt="微信圖片_20200814122753" style="zoom: 25%;" />
<img src="C:\Users\Lenovo\Desktop\iI\未標題-1.png" style="zoom:10%;" />
