《Functional Proteomics Identifies Acinus L as a Direct Insulin- and Amino Acid-Dependent Mammalian Target of Rapamycin Complex 1 (mTORC1) Substrate》
We confirmed acinus L as a direct mTORC1 substrate by co-immunoprecipitation and MS-enhanced kinase assays. Our study delineates a triple proteomics strategy of combined phosphoproteomics, interactomics, and MS-enhanced kinase assays for the de novo-identification of mTOR network components, and provides a rich source of potential novel mTOR interactors and targets for future investigation.
1.是幻燈片整理的那篇文章栅葡。
- 采用 phosphoproteomics和 interactomics的交集库正，選出acinus L進(jìn)行分析從而判定acinus L as a direct mTORC1 substrate。其依據(jù)的假設(shè)是proteins whose phosphorylation depends on the presence of insulin?aa and/or mTORC1, and
that interact with mTOR have a high probability to be direct mTORC1 substrates. - 我的研究計(jì)劃3）和4）的思路都出自這篇文章衫生。
