腸道細(xì)胞類型及其marker:
seven known cell types were identified (Fig. 1, A and B):
enterocyte cells (ALPI, SLC26A3, TMEM37, and FABP2),
goblet cells (ZG16, CLCA1, FFAR4, TFF3, and SPINK4),
PCs (LYZ [Lyz1 and Lyz2 in mouse], CA7, SPIB, CA4, and FKBP1A),
enteroendocrine cells (CHGA, CHGB, CPE, NEUROD1, and PYY),
progenitor cells (SOX9, CDK6, MUC4, FABP5, PLA2G2A, and LCN2),
transient-amplifying (TA) cells (KI67, PCNA, TOP2A, CCNA2, and MCM5), and
stem cells (LGR5, RGMB, SMOC2, and ASCL2).
Using the same cell markers (Fig. S2 B), these cell types were also identified in the ileum, colon, and rectum segments when analyzed separately (Fig. 1, C–H; and Table S2). Tuft cell markers (POU2F3, GFI1B, and TRPM5) were rarely detected in few cells (Fig. S2 C), while the marker DCLK1 was not detected.
細(xì)胞類型特異的轉(zhuǎn)錄因子：
We have also observed specific expression of transcription factors in different types of cells (CREB3L3, MAF, and NR1H4 in enterocytes; ATOH1, SPEDF, and FOXA3 in goblet cells; SPIB, HES4, and PROX1 in PCs and PLCs; FEV, INSM1, and NEUROD1 in enteroendocrine cells; YBX1 and PHB in both TA and stem cells; HMGB2, FOXM1, and MYBL2 in TA cells; and ASCL2 and ETS2 in stem cells; Fig. S3 F). These cell type–dependent transcription factors may play important roles in the differentiation or maintenance of the different cell types. Indeed, INSM1 has been shown to be essential for enteroendocrine cell differentiation (Gierl et al., 2006), and ASCL2 is required for the maintenance of intestinal stem cell identity (Schuijers et al., 2015).
https://rupress.org/jem/article/217/2/e20191130/132578/Single-cell-transcriptome-analysis-reveals
