本以為在佛前

卻不想那不是我的佛

墮落于塵

手捧著一杯濁酒

敬你 敬曾相遇

彼岸花的馨香已伴著河燈起舞

孟婆那一碗一碗的湯

將我喚起 又要走過一世的記憶

長生殿里 你說你不想再來了

菩提樹下 你說你忘了如何回家了

轉輪塔中 你說你還記得 記得那一世

梵音終啟? 一切歸于沉寂

叫一聲佛主? 隨風而去

因為那個你就是我呀

一切都是一場自編自導的戲

終要有一幕絕妙的結局

Application of high performance liquid chromatograph (HPLC)

HeJiao1

1.College

of food and Bioengineering, Henan University of science and technology,

Luoyang, 471003

Abstract:

Highperformance liquid chromatograph correlation analysis is the main separationand analysis technology in biology, chemistry and medicine. It plays anindispensable role in food inspection, scientific research, environmentalmonitoring, drug development, production and drug inspection. HPLC refers tothe theory of meteorological chromatography on the basis of the originalclassical chromatography, and the chromatographic column is filled with smallparticles in a special way. The column efficiency of chromatographic column ismuch higher than that of classical liquid chromatography. After the column isused, it can also have a high sensitivity detector, which can continuouslydetect the analyte coming out of the convection. High performance liquid chromatographis simple in operation and wide in application. It can be used for accurateanalysis of experimental articles, making the detection results more accurateand improving the detection accuracy. This paper reviews the practicalapplication of HPLC.

Keywords:HPLC;application; analysis

Introduction

Highperformance liquid chromatograph (HPLC) is composed of chromatography andcontroller, injector, column pump, data processor, detector and control center.The system is mainly composed of injection system, infusion system, separationsystem, detection system and data processing system. HPLC is a kind ofchromatography analysis with high separation efficiency, fast analysis speedand automatic operation developed in 1960s [1]. HPLC is an important branch ofchromatography. Its working principle is to enter mobile phases such as singlesolvent with different polarity or mixed solvent with different proportion,buffer solution, etc. into the column with fixed phase through high-pressurepump. After the components in the column are separated according to the orderof polarity, they enter the detector for detection, so as to realize theanalysis of samples [2]. According to statistics, about 20% of known compoundscan be analyzed by gas chromatography, and about 70% ~80% can be analyzed byliquid chromatography. HPLC has a wide range of applications. It can beflexibly used in food inspection, scientific research, food and druginspection, environmental monitoring and other processes. The analysis andresearch of its application is of scientific significance.

1. Applicationof HPLC in drug identification and analysis

The

application of HPLC in drug identification is also an important work in the

medical industry. If the quality of drug identification cannot be guaranteed,

the whole medical industry will be seriously affected. Generally speaking,

there is little difference between the appearance of fake drugs or mixed drugs

and real drugs. It is difficult for ordinary people to identify them. However,

the performance of drugs is far behind. Therefore, the most advanced technology

must be used for identification. HPLC is a reliable method for drug

identification[3].

Highperformance liquid chromatograph is used to determine the content of drugs,which can ensure the accuracy of the drugs used, and is conducive to the cureof the disease. For example, the pharmaceutical composition of Chinesetraditional medicine is relatively complex. Animals, plants and minerals canall be used. Therefore, it is very difficult to promote it internationally,because it is impossible to prove what is the effective composition. Therefore,accurate measurement of the content of drugs can improve the production qualityand use value of traditional Chinese medicine. Using HPLC to determine thecontent of natural drugs can effectively ensure the application effect of drugsin China, which is not only conducive to the production of drugs, but alsoconducive to the cure of diseases.

2. Applicationof HPLC in food detection

With the development of time, people's livingstandards have improved, and Chinese people pay more and more attention to foodsafety. The instruments and corresponding methods for food safety detectionhave also attracted the attention of scientists all over the world. HPLC hasbecome the most important food detection technology because of its highsensitivity and low detection limit [4].

For example, check vitamin B1 in food. According to

the literature, Chen Chunxiao, Zhang Huimin, Zhong Yuetong and Kang Li have

used the combination of on-line derivatization technology and fluorescence

detector to determine the food method: use the on-line derivatization function

of HPLC to convert vitamin B1 in food into thiamine fluorescence, and then use

the fluorescence detector to detect its content[5]. HPLC was used flexibly in this method, and the

detection purpose was achieved quickly. Plants are rich in vitamins, especially

B vitamins, which play an important role in nutrition, food safety, health and

therapeutic effect[6]. Barbecue and other cooking technologies are loved

by food lovers at home and abroad. However, barbecue can lead to the formation

of harmful compounds, such as HCAs. Heterocyclic amine is a strong carcinogenic

and mutagenic nitrogen-containing compound, which produces protein rich food

under certain cooking conditions[7]. Charles F. manful, Natalia P and others used HPLCto analyze them, revealing the harmful substances produced by barbecue, whichis of positive significance to guide people to master barbecue and reduce thegeneration of harmful substances. HPLC plays an important role in fooddetection. It has many advantages, such as many kinds of mobile phases, highsensitivity, automation and wide application.

3. Applicationof HPLC in environmental monitoring

Green water and green mountains are golden

mountains and silver mountains. Everyone from the country to the country

attaches great importance to environmental issues. It is of course very

important to determine an instrument that can analyze and monitor environmental

problems timely and accurately. In the history, environmental pollution

disasters happened repeatedly. In 1950, a synthetic acetic acid plant in

Minamata Town, Japan, used mercuric chloride and mercuric sulfate as catalysts

in its production and discharged them into the nearby Minamata Bay at the same

time, thus entering the food pond and converting them into harmful organic

mercury compounds such as methylmercury chloride, which caused local residents

to suffer from neurological diseases. It is precisely because of the occurrence

of this famous pollution event that the concept of elemental morphological

analysis is first proposed [8-10]. Speciation analysis of elements is very important in environmental and

biological analysis, and it has become the research focus of environmental and

biological analysis. Various high-performance separation technologies, such as

high-performance liquid chromatography, GC and capillary electrophoresis (CE),

are widely used in environmental analysis[11]. The most

elements involved in the analysis of element form in environmental detection

include arsenic, selenium, mercury, chromium, etc. these studies are widely

used in the construction of national or regional evaluation system. The

traditional pollutants in water resources are mainly defined as special

elements and nutrient elements. For these elements, it is difficult to detect

and distinguish with ordinary detection instruments. The application of HPLC

can distinguish these elements in detail, and accurately detect various

substances contained in these elements in water resources[12,13]. The role

of HPLC in the detection of water resources and its future development

prospects provide a new direction for improving water resources and environment

in China[14]. From this we can see the importance of HPLC inenvironmental monitoring.

4. Applicationprospect of HPLC

It can be seen from the above that HPLC plays an

important role in drug identification and analysis, food safety detection and environmental

monitoring. At present, there are many problems in these three aspects at home

and abroad[15,16]. Therefore, the application scope of HPLC should

be further expanded to further improve the detection ability. To better meet

the requirements of detection, HPLC in the use of the process, with a certain

continuous injection ability to combine with the injection function of

automatic operation will achieve good results[17].. In the process of automatic operation, the

injection accuracy will be affected, and the injection efficiency is low. In

order to change this phenomenon, the continuous injection device and HPLC can

be used together, which can not only improve the efficiency of injection, but

also realize the automatic operation of the whole process, improve the accuracy

of injection[18]. When using high performance liquid chromatograph

for detection, the detection efficiency can be effectively guaranteed[19]. When mass spectrometer and HPLC detector are used

together, the unknown samples can be analyzed in detail in the process of

sample detection. HPLC has the function of data analysis and calculation. When

HPLC and evaluation software are used together, the function of data

calculation and analysis of HPLC can be maximized[20].

It can be seen that the application prospect ofHPLC is very broad, not only limited to the several aspects listed in thispaper, but also more application directions are waiting for development.

Conclusion

After continuous practice and research, HPLC hasbeen widely used. In the process of analyzing the scope of its application, wecan see the effect of HPLC in practical application. According to the testresults, we can also see that HPLC has many advantages, such as high detectionefficiency, simple operation, wide application range et al.

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