腳本位置：/home/shen/biosoft/HiC-Pro_2.11.1/bin/utils
1.digest_genome.py
## Digest the mm9 genome by HindIII
HICPRO_PATH/bin/utils/digest_genome.py -r A^AGCTT -o mm9_hindiii.bed mm9.fasta
## The same ...
HICPRO_PATH/bin/utils/digest_genome.py -r hindiii -o mm9_hindiii.bed mm9.fasta
## Double digestion, HindIII + DpnII
HICPRO_PATH/bin/utils/digest_genome.py -r hindiii dpnii -o mm9_hindiii_dpnii.bed mm9.fasta
"mboi": ["^GATC"],
"dpnii": ["^GATC"],
"bglii": ["A^GATCT"],
"hindiii": ["A^AGCTT"]}
2.hicpro2fithic.py
python /home/shen/biosoft/HiC-Pro_2.11.1/bin/utils/hicpro2fithic.py -i raw/100000/TU-2_100000.matrix -b raw/100000/TU-2_100000_abs.bed -s iced/100000/TU-2_100000_iced.matrix.biases
生成三個(gè)文件：
228K? ? fithic.biases.gz
144K????fithic.fragmentMappability.gz
2.7M????fithic.interactionCounts.gz
接著：
ref：https://github.com/ay-lab/fithic#-x---chromosome_region
pip install fithic
fithic -h?
報(bào)錯(cuò)：“No module named functools_lru_cache” 解決：在普通py環(huán)境下：pip install arrow==0.12.0
運(yùn)行：
fithic -f fithic.fragmentMappability.gz -i fithic.interactionCounts.gz -t fithic.biases.gz -o fithic -l TU-2 -v -x intraOnly -r 100000
-l lable
-v 可視化
-x?intraOnly?interOnly?All
-r, --resolution
他的文章：https://genome.cshlp.org/content/24/6/999
3.hicpro2higlass.sh
bash?/home/shen/biosoft/HiC-Pro_2.11.1/bin/utils/hicpro2higlass.sh -h
chrsize=/media/shen/*/sun/refdata/UCSC_mm10/mm10.chrom.sizes
bash /home/shen/biosoft/HiC-Pro_2.11.1/bin/utils/hicpro2higlass.sh -i raw/100000/TU-2_100000.matrix -r 100000 -c $chrsize -n?
生成兩個(gè)文件：
接著： 可以用HiGlass瀏覽器來(lái)看。
ref：http://gehlenborglab.org/research/projects/higlass/
4.hicpro2juicebox.sh
bash?/home/shen/biosoft/HiC-Pro_2.11.1/bin/utils/hicpro2juicebox.sh -h
bash /home/shen/biosoft/HiC-Pro_2.11.1/bin/utils/hicpro2juicebox.sh -i TU-2/TU-2.allValidPairs -g /home/shen/biosoft/HiC-Pro_2.11.1/annotation/chrom_mm10.sizes -j /media/shen/*/sun/biosoft/juicer_tools.jar -r /home/shen/biosoft/HiC-Pro_2.11.1/annotation/mm10.mbo1.bed
!!! 注意：這里需要矯正舍肠？
bash /home/shen/biosoft/HiC-Pro_2.11.1/bin/utils/hicpro2juicebox.sh \
-i TU-2/TU-2.allValidPairs \ validpairs
-g /home/shen/biosoft/HiC-Pro_2.11.1/annotation/chrom_mm10.sizes \ 基因組size信息
-j /media/shen/*/sun/biosoft/juicer_tools.jar \ juicer包
-r /home/shen/biosoft/HiC-Pro_2.11.1/annotation/mm10.mbo1.bed # 限制酶切文件
生成：hic文件
juicerbox：
java -jar /media/shen/*/sun/biosoft/Juicebox_1.9.8.jar
5.sparseToDense.py
python /home/shen/biosoft/HiC-Pro_2.11.1/bin/utils/sparseToDense.py -h
python /home/shen/biosoft/HiC-Pro_2.11.1/bin/utils/sparseToDense.py iced/100000/TU-2_100000_iced.matrix -b raw/100000/TU-2_100000_abs.bed -d -o TU-2.matrix
接著：
Create the DI file：
chrsize=/home/shen/biosoft/HiC-Pro_2.11.1/annotation/chrom_mm10.sizes
/home/shen/biosoft/domaincall_software/perl_scripts/DI_from_matrix.pl?TU-2.matrix 100000 200000 $chrsize > TU-2.matrix.di
修改DI文件：(mouse)
cat?TU-2.matrix.di | grep -v?"M" |?sed -e 's/^X/20/g' |?sed -e 's/^Y/21/g' >?TU-2.matrix.fine.di?
cp /home/shen/biosoft/domaincall_software/HMM_calls.m ./
編輯：HMM_calls.m文件：
Note:
[1]In line 9 of the script HMM_calls.m,pleasehardcodeyour inputDIfilename.
[2]In line 77 of the script HMM_calls.m,pleasehardcodeyour outputfilename.
nice matlab < HMM_calls.m > dumpfile
# 用dekker的2015perl腳本：
ref：?https://github.com/dekkerlab/crane-nature-2015
perl /home/shen/biosoft/crane-nature-2015-master/scripts/matrix2insulation.pl -i TU-2.matrix
6.R包：HiTC
library("HiTC")
x=importC("../rawdata_40000_iced.matrix", xgi.bed="../rawdata_40000_ord.bed")
R=extractRegion(x$chr1chr1, chr="chr1", from=1, to=249250621) #chesize
di<-directionalityIndex(R)
ord=read.delim(paste("rawdata_40000_ord.chr1.cut.bed",sep="."), header=F)
RM=as.matrix(intdata(R))
RF=cbind(ord[,c(1:3)], RM)
ord$DI=di
ord$V1=paste("1")
ord$DI[is.na(ord$DI)]<-0
ord$DI[is.nan(ord$DI)]<-0
FIN.DI=ord[,c(1,2,3,4)]
=> RF is the matrix from HiTC, FIN.DI is the DI file from HiTC
