2020.02.04文獻(xiàn)
- 1. 3,3-Dimethyl-1-butanol attenuates cardiac remodeling in pressure-overload-induced heart failure mice. by Guangji Wang, Bin Kong, …, He Huang (2020) J. Nutr. Biochem. (impact factor: 4.5) 2 區(qū)
P (研究人群/動(dòng)物) C57BL6/J male mice (8-10 weeks old)
I（干預(yù)措施) AB+with or without 1% DMB for 6 weeks
C (對(duì)照措施) Sham+with or without 1% DMB for 6 weeks
O (結(jié)果)Cardiac function, plasma TMAO level, cardiac hypertrophy and fibrosis, expression of inflammatory, susceptibility to ventricular arrhythmia and p65 NF-κB and TGF-β1/Smad3 signaling pathway were prevented by DMB treatment at the 6th week after AB surgery
思考
- 心功能只有射血分?jǐn)?shù)、血液動(dòng)力學(xué)指標(biāo)緩解了,但是病理指標(biāo)都緩解了灾而，可靠否屉来？
- 機(jī)制也是只觀察了現(xiàn)象
- 沒(méi)有細(xì)胞實(shí)驗(yàn)
- 2. DNMT1 Methylation of LncRNA GAS5 Leads to Cardiac Fibroblast Pyroptosis via Affecting NLRP3 Axis. by Qian She, Peng Shi, …, Yan Yang (2020) Inflammation (impact factor: 2.9)3 區(qū)
P In Vivo: Sprague-Dawley rats weighing 180 to 200 g; In Vitro: SD rats of 3–5 days old
I In Vivo: ISO 5 mg/kg, for 2 weeks;In Vitro: 1 μg/ml of LPS for 5.5 h, and then cultured for 5 h by adding 5 ng/ml of ATP
C In Vivo: normal saline for 2 weeks; In Vitro :PBS
O DNMT1 methylation silencing of LncRNA GAS5 could lead to cardiac fibroblast pyroptosis
and activated the NLRP3, thus promoting the progression of cardiac fibrosis.
思考
- LncRNA與甲基化就是一個(gè)siRNA和一個(gè)甲基酶抑制劑就證明有相互關(guān)系了嗎雷酪？
- 敲低或者過(guò)表達(dá) LncRNA GAS5 的時(shí)候?yàn)槭裁礇](méi)有LPS+敲低或者過(guò)表達(dá)組,只有Control NC Si/PcNDA組?
- 3. Inhibiting SLC26A4 reverses cardiac hypertrophy in H9C2 cells and in rats. by Liqun Tang, Xiaoqin Yu, Yangyang Zheng, Ning Zhou (2020) PeerJ (impact factor: 2.4) 3 區(qū)
P In Vivo:ten healthy male Sprague Dawley (SD) rats weighed 250 ± 20 g In Vitro: H9C2 cells (an embryonic rat myocardium-derived cell line)
I In Vivo: abdominal aortic constriction (AAC)+injected with or without siRNA-SLC26A4 (2.5 ul) once a day for seven days.; In Vitro:PE (200 mM) for 48 h+SLC26A4 siRNA or the control siRNA
C In Vivo: Sham+ normal saline for for seven days; In Vitro :PBS?++SLC26A4 siRNA or the control siRNA
O Inhibiting SLC26A4 could decrease the release of ANP or BNP and promote the expression of GSK-3beta in vivo and in vitro.
思考
- 動(dòng)物試驗(yàn)中沒(méi)有sham+siRNA組
- 動(dòng)物試驗(yàn)中沒(méi)有心功能緩解的情況.只有個(gè)Mrna和病理緩解情況
- 結(jié)論中"SLC26A4 increases cardiac hypertrophy,"文中并沒(méi)有做過(guò)表達(dá)實(shí)驗(yàn),如何得到這個(gè)結(jié)論?
- GSK-3beta這個(gè)指標(biāo)能夠提到beta-MHC?
- 4. The Pro-arrhythmic Features of Pathological Cardiac Hypertrophy in Neonatal Rat Ventricular Cardiomyocyte Cultures. by Zeinab Neshati, Martin Schalij, Antoine de Vries (2020) J. Appl. Physiol.
跟我們的研究好像沒(méi)啥關(guān)系,沒(méi)看了
PS
