1.打開軟件--Fasttools----》Fast Extract(recommended)
2. 建立基因組索引
3. 設(shè)置輸出數(shù)據(jù)路徑
4.設(shè)置輸入數(shù)據(jù)扣汪,\t f分開。
示例:MSU
LOC_Os10g33000
ATGGTGGAAGAAAGAGAAAGCTTCGAGAGTCAGTGGGCGCCCTCCGATGTGACAGAAGAT
AATCTGAAGGAGATGGTGGCGCACGGCGTTCTTCCAGCGAAGGAGATCATCGGGTGGCGA
CCGGCATTTGGCGAAGCATTCCCGACTCCCGATACGCATGAAATCGTGGTATTTGCTCAC
TTCTTCTATGGCGGATTTTCTCTTCCAACCTCAAGATTTTTCCGGGGCATTCTGAACTTC
TACGGGATTAGCTTGCATCATCTGAATCCAAACTCCATAGTGCATATTGCCAATTTTATC
CACGCCTGCGAAGCTTTTCTGGGCATTAGGCCTCATTTTGCCTTGTTCCGCCGCATCTTC
提取的數(shù)據(jù):
Chr10 17276945 17279876
ATGGTGGAAGAAAGAGAAAGCTTCGAGAGTCAGTGGGCGCCCTCCGATGTGACAGAAGAT
AATCTGAAGGAGATGGTGGCGCACGGCGTTCTTCCAGCGAAGGAGATCATCGGGTGGCGA
CCGGCATTTGGCGAAGCATTCCCGACTCCCGATACGCATGAAATCGTGGTATTTGCTCAC
TTCTTCTATGGCGGATTTTCTCTTCCAACCTCAAGATTTTTCCGGGGCATTCTGAACTTC
TACGGGATTAGCTTGCATCATCTGAATCCAAACTCCATAGTGCATATTGCCAATTTTATC
CACGCCTGCGAAGCTTTTCTGGGCATTAGGCCTCATTTTGCCTTGTTCCGCCGCATCTTC