在該頁(yè)面登記后會(huì)彈出下載鏈接：http://weatherby.genetics.utah.edu/cgi-bin/registration/maker_license.cgi
1. 安裝perl包
source activate maker
conda install perl-inline-c perl-perl-unsafe-signals perl-want perl-forks perl-dbd-pg
2. 配置mpi
用conda安裝mpich2清笨，我這里安裝的版本是mpich2-1.4.1p1舷丹。
conda install mpich2=1.4.1p1
我這里使用的是mpich2验毡，用openmpi不是很好使蒿偎。
3. 安裝依賴軟件
3.1 安裝RMBlast
wget -c http://www.repeatmasker.org/rmblast-2.11.0+-x64-linux.tar.gz
#添加環(huán)境變量
echo "export PATH=/home/chenhx/software/rmblast-2.11.0/bin:"'$PATH' >> ~/.bashrc
3.2 安裝snap
wget -c http://korflab.ucdavis.edu/Software/snap-2013-11-29.tar.gz
tar -zxvf snap-2013-11-29.tar.gz
make
echo "export PATH=/home/chenhx/software/snap:"'$PATH' >> ~/.bashrc
echo "export Zoe=/home/chenhx/software/snap/Zoe:"'$PATH' >> ~/.bashrc
3.3 安裝RepeatMasker
見(jiàn)“RepeatModeler + RepeatMasker安裝”篇
3.4 安裝Exonerate 2.2
wget -c http://ftp.ebi.ac.uk/pub/software/vertebrategenomics/exonerate/exonerate-2.2.0-x86_64.tar.gz
tar -zxvf exonerate-2.2.0-x86_64.tar.gz
echo "export PATH=/home/chenhx/software/exonerate-2.2.0-x86_64/bin:"'$PATH' >> ~/.bashrc
3.5 安裝Augustus
conda install Augustus
3.6 安裝genemark-ES
下載genemark-ES，軟件安裝包可以自行下載http://exon.gatech.edu/GeneMark/license_download.cgi。
tar -zxvf gmes_linux_64.tar.gz
cp gm_key_64 ~/.gm_key
echo "export PATH=/home/chenhx/software/gmes_linux_64:"'$PATH' >> ~/.bashrc
3.7 安裝maker
下載地址http://weatherby.genetics.utah.edu/cgi-bin/registration/maker_license.cgi
conda install perl-dbd-sqlite perl-io-all perl-bit-vector perl-Bio-Procedural
tar -zxvf maker-2.31.11.tgz
cd maker/src
perl Build.PL
#編譯時(shí)選擇不用mpi，默認(rèn)是N佑女，直接回車即可
./Build install
echo "export PATH=/home/chenhx/software/maker/src/bin:"'$PATH' >> ~/.bashrc
