一天的工作結束了秤朗，今天過的很好煤蹭。因為沒有值班娇昙，所以一天的時間都花在文獻閱讀上面窒舟。在昨天的基礎上看完了一篇文獻，并且按照網上搜索的方法進行做了筆記。以前都是用本子來記彼宠，但今天沒有硼讽，而是見了一個word的文檔鸳惯，并命名為《文獻與讀筆記》鸦做，希望這個文檔能堅持續(xù)寫幾年。在此一在這里將復制一下今天的筆記贝或，一此來紀念新的閱讀方式吼过。
title 《Association study of gut flora in coronary heart disease through high-throughput sequencing》
Introduction
Although the diversity of human gut microbiota has been observed in different individuals, the unique core of its composition is stable, suggesting a potiental risk of intestinal microbiota for human disease. Relationships between gut microbiota and cardiovascular disease have attracted more and more attentions. Oral dietary supplementation with L-oxide by gut microbiota, has been proved to accelerate atherosclerosis in mice. Then, high-throughput sequencing was used to assess the taxonomic composition of the microbiota in CHD patients.
Materials and methods
Between April 2015 and June 2015,29 CHD in hospital patients were recruited and 35 healthy volunteers were recruited as control. The inclusion criteria as follows (1,2,3). Fresh fecal samples(2-5g) were obtained from all the patients under the hospital diet and controls under the usual diet.
Results
3.1 baseline characteristics of patients. Age, sex, BMI, hypertension.
3.2 high-throughput sequencing data analysis.
3.3 OTU analysis. venn picture and rank-abundance curve of operational taxonomic units in the groups.
3.4 specials classification. Reads corresponding to 38 phyla, 90 classes, and 584 genera were detected in CHD patients fecal samples.
3.5 analysis of a Diversity index. a diversity of the CHD patients microbiota was significantly higher than that of. The controls. Chao 1 index, and Ace index in the CHD patients were higher than those in the control group.
3.6 Intestinal flora structure analysis.
3.7 PcoA and UPGMA TREE analysis. Samples contribution rates of the first the PcoA second PcoA(PC2), and third PcoA (PC3) were 11.85%,4.09%,and 2.84% respectively, which highlighted a clear clustering of the microbial population of the CHD patients away from that of the controls.
DISCUSSION
Obesity is one of the major risk factors for CHD, as the process from overweight to obese brings in a large number of comorbidities, which are harmful for cardiovascular health. As an independent maker of the risk of cardiovascular disease, blood proteobacteria has been verified to be positively related to the onset of cardiovascular complications. Same limitations. First, the samples size of patients was small. Second, it is unclear whether the differences of gut flora compositions between groups were response to CHD or they actively inducted the CHD.
明天后天兩天將會休息，而明天將準備騎著共享單車去40公里之外的響水澗看看油菜花咪奖，當然這一切是在明天有個好天氣的情況下盗忱。期待明天的獨自旅行。
