An overview of ChIP–seq, DNase-seq, ATAC-seq, MNase-seq and FAIRE–seq experiments.
看到六六博客有不錯(cuò)的骄蝇，故直接轉(zhuǎn)載：
- ATAC-Seq膳殷、ChIP-Seq、Dnase-Seq乞榨、MNase-Seq秽之、FAIRE-Seq整體的分析思路一致当娱，找到富集區(qū)域吃既，對(duì)富集區(qū)域進(jìn)行功能分析。
- ChIP-Seq是揭示特定轉(zhuǎn)錄因子或蛋白復(fù)合物的結(jié)合區(qū)域跨细，實(shí)際是研究DNA和蛋白質(zhì)的相互作用鹦倚，利用抗體將蛋白質(zhì)和DNA一起富集，并對(duì)富集到的DNA進(jìn)行測(cè)序冀惭。
DNase-Seq震叙、ATAC-Seq、FAIRE-Seq都是用來(lái)研究開放染色質(zhì)區(qū)域散休。
- DNase-Seq是用的DNase I內(nèi)切酶識(shí)別開放染色質(zhì)區(qū)域媒楼，而ATAC-seq是用的Tn5轉(zhuǎn)座酶，隨后進(jìn)行富集和擴(kuò)增戚丸；
- FAIRE-Seq是先進(jìn)行超聲裂解划址，然后用酚-氯仿富集。
- MNase-Seq是用來(lái)鑒定核小體區(qū)域限府。
作者：六六_ryx
鏈接：http://www.reibang.com/p/87bc2002e82c
來(lái)源：簡(jiǎn)書
圖片來(lái)源：
Identifying and mitigating bias in next-generation sequencing methods for chromatin biology
摘要：
Next-generation sequencing (NGS) technologies have been used in diverse ways to investigate various aspects of chromatin biology by identifying genomic loci that are bound by transcription factors, occupied by nucleosomes or accessible to nuclease cleavage, or loci that physically interact with remote genomic loci. However, reaching sound biological conclusions from such NGS enrichment profiles requires many potential biases to be taken into account. In this Review, we discuss common ways in which biases may be introduced into NGS chromatin profiling data, approaches to diagnose these biases and analytical techniques to mitigate their effect.
