單端測序文件
for i in *IP*fastq; do
?~/Software/sortmerna-2.1b/sortmerna --ref /home/zhou/Software/sortmerna-2.1b/index/human_rRNA.fa,/home/zhou/Software/sortmerna-2.1b/index/human_rRNA_db: --reads $i --num_alignments 1 --fastx --aligned reads_rRNA --other ${i}_rmrRNA.fq --log -a 8 -m 24000 -v;
?done
雙端測序
合并read?files
/path_to_sortmerna/scripts/merge-paired-reads.sh reads_1.fq reads_2.fq reads_interleaved.fq
去除rRNA
?~/Software/sortmerna-2.1b/sortmerna --ref /home/zhou/Software/sortmerna-2.1b/index/human_rRNA.fa,/home/zhou/Software/sortmerna-2.1b/index/human_rRNA_db: --reads $i --num_alignments 1 --fastx --aligned reads_rRNA --other ${i}_rmrRNA.fq --log -a 8 -m 24000 --paired_in -v;
分解merged的read?flie
/path_to_sortmerna/scripts/unmerge-paired-reads.sh reads_non_rRNA.fq reads_non_rRNA_1.fq reads_non_rRNA_2.fq
