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  • 請問老師構(gòu)建數(shù)據(jù)庫的時候報錯如何解決奢讨?我輸入的命令是kraken2-build --standard --threads 24 --db $DBNAME
    Downloading nucleotide gb accession to taxon map...rsync: failed to connect to ftp.ncbi.nlm.nih.gov (2607:f220:41e:250::10): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (2607:f220:41e:250::13): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (130.14.250.7): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (165.112.9.229): Network is unreachable (101)
    rsync error: error in socket IO (code 10) at clientserver.c(125) [Receiver=3.1.2]

    Kraken2安裝與數(shù)據(jù)庫構(gòu)建2022-01-02

    Kraken2是一款快速宏基因組DNA序列進(jìn)行物種注釋的軟件稚叹。我將其用于測序數(shù)據(jù)以及序列的物種的物種注釋,檢查測序數(shù)據(jù)以及序列是否有污染禽笑。conda以及github網(wǎng)址:ht...

  • 請問構(gòu)建數(shù)據(jù)庫的時候報錯如何解決入录?
    Downloading nucleotide gb accession to taxon map...rsync: failed to connect to ftp.ncbi.nlm.nih.gov (2607:f220:41e:250::10): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (2607:f220:41e:250::13): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (130.14.250.7): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (165.112.9.229): Network is unreachable (101)
    rsync error: error in socket IO (code 10) at clientserver.c(125) [Receiver=3.1.2]

    如何構(gòu)建kraken2個性化數(shù)據(jù)庫

    kraken2就不介紹了,是一款挺好用的快速比對宏基因組軟件佳镜。 現(xiàn)在有了nt數(shù)據(jù)庫下面animal的序列僚稿,那么如何構(gòu)建kraken2的數(shù)據(jù)庫呢? 首先推薦把kraken2安裝...

  • ~/software/Bracken/bracken-build -d ~/db/minikraken2_v1_8GB -t 8 -k 35 -l 150
    這個命令會報錯ERROR: Database library ~/kraken2/minikraken2_v1_8GB/library does not exist
    請問怎么解決呀蟀伸?

    Kraken2+Bracken

    1. 簡介 Kraken2是一個基于k-mer算法的高精度宏基因組序列分類軟件蚀同,能夠快速的將測序reads進(jìn)行物種分類。 Kraken2官網(wǎng)[http://ccb.jhu.e...

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