資料源自解螺旋
Long Non-Coding RNA (LncRNA) HOXA11-AS Promotes Breast Cancer Invasion and Metastasis by Regulating Epithelial-Mesenchymal Transition
- 疾 breast cancer
- 型 invasion and metastasis EMT
- 模 [method]
- 法 [method]
- 標(biāo) HOXA11
分子來(lái)源
Recent studies have revealed that HOXA11-AS could serve as an important biological marker and therapeutic target for other types of tumors [13–15]. However, there is no report about the role of lncRNA HOXA11-AS in breast cancer
In recent years, many studies have reported that lncRNA is involved in tumor invasion and metastasis by regulating EMT. Hao et al. [21] found that, as an important regulatory factor, lincRNA 01186 was involved in lung cancer cell invasion and metastasis by regulating EMT. Additionally, our results revealed interference in lncRNA HOXA11-AS could result in changes in the expressions of EMT molecular markers such as E-cadherin, N-cadherin, and Vimentin.
沒(méi)做回復(fù)Rescue實(shí)驗(yàn)（沒(méi)機(jī)制沒(méi)法Rescue）
實(shí)驗(yàn)方法
- Breast cancer tissue specimens
- Breast cancer cell strains
- Primer synthesis of siRNA and qRT-PCR interfering in
HOXA11-AS - Detection of HOXA11-AS expression via real-time
fluorescence quantification PCR - Cell culture and the transfection of human breast cancer
cell strain - Detection of breast cancer cell strain proliferation via MTT
assay and clone formation assay - Detection of cell cycle and apoptosis changes via flow
cytometry - Detection of cell migration and invasion capacity changes
via Transwell experiment and wound healing assay - Detection of EMT-related molecular marker expression
changes via Western blotting and immunofluorescence
experiment - Research on the influence of HOXA11-AS on tumor cell
metastasis capacity via experiment in vivo - Immunohistochemistry
結(jié)果
- Up-regulated expression of HOXA11-AS in breast cancer
tissue and cells
表達(dá)差異 - Research on the biological function of HOXA11-AS in
breast cancer cell via experiment in vitro
體外實(shí)驗(yàn) - Research on the influence of HOXA11-AS on breast cancer cell invasion and metastasis via in vivo experiment
體內(nèi)實(shí)驗(yàn) - HOXA11-AS promotes breast cancer cell invasion and
metastasis by regulating EMT
圖片解析
A：癌癥病人中表達(dá)
B：細(xì)胞株表達(dá)
C崎脉、D：轉(zhuǎn)染沉默基因表達(dá)情況
A豌骏、B：兩個(gè)不同細(xì)胞株檢測(cè)沉默基因后的生長(zhǎng)曲線（MTT）
C：兩個(gè)不同細(xì)胞株檢測(cè)沉默基因后的克隆形成
D：對(duì)C圖的統(tǒng)計(jì)
A：流式細(xì)胞檢測(cè)沉默基因后凋亡情況
B：對(duì)A的統(tǒng)計(jì)
C宛渐、D：沉默基因后細(xì)胞周期變化
A/B/C/D：基因沉默后的侵襲苏遥、增殖能力變化
為什么轉(zhuǎn)移后表達(dá)反而低了厕怜？侮东？墩瞳？圖片中sh應(yīng)該是si
N-cadherin或E-cadherin是EMT的biomarker
圖片中sh應(yīng)該是si
