教程在：腫瘤外顯子數(shù)據(jù)處理系列教程（一）讀文獻并且下載測序數(shù)據(jù) (qq.com)
點開
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里面會有很多后續(xù)的分析
Reliability of Whole-Exome Sequencing for Assessing Intratumor Genetic Heterogeneity - ScienceDirect文章鏈接
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數(shù)據(jù)下載
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NCBI的Sequence Read Archive (SRA)，每個項目的url格式都是一樣的，https://www.ncbi.nlm.nih.gov/Traces/study/?acc=SRPXXX
https://www.ncbi.nlm.nih.gov/Traces/study/?acc=SRP070662
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我們只需要下載wes的
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下載好了枉长，我是自己電腦上建了一個目錄D:\work\腫瘤外顯子數(shù)據(jù)分析學(xué)習(xí)
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runtable
acclist
RunInfo Table: 包含了較多的信息蠢箩，可用于數(shù)據(jù)下載完成后對文件的重新命名
Accesssion List: 只有一列杆查，prefetch可以接受改文件也殖，下載列表中包含的所有樣本
下載數(shù)據(jù)使用的軟件是prefetch既绕，SRA Toolkit的套件之一缴挖，如果使用conda的話袋狞，需要安裝的軟件是sra-tools，而不是prefetch醇疼。
首先檢查一下有沒有這個軟件
11:08:26 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES
$
prefetch -help
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首先建立一個命名為wes的conda環(huán)境
## 組織項目
mkdir 0.sra log
## 安裝conda
#wget https://repo.anaconda.com/miniconda/Miniconda2-latest-Linux-x86_64.sh
#bash Miniconda2-latest-Linux-x86_64.sh
## 使用官方鏡像
conda config --add channels conda-forge
## 創(chuàng)建一個wes環(huán)境
conda create -n wes python=2
conda info --envs
## 創(chuàng)建后需要激活該環(huán)境
source activate wes
## 所有的安裝都是建立在該環(huán)境已經(jīng)激活的前提下硕并，后續(xù)使用到的軟件，同樣需要激活后再安裝
#conda install sra-tools
這個腳本按照自己需求更改
11:15:48 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES
$
mkdir run
11:15:58 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES
$
cd run
11:16:01 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
vim creat-wes-envs.sh
11:17:26 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
sh creat-wes-envs.sh
Warning: 'conda-forge' already in 'channels' list, moving to the top
Collecting package metadata (current_repodata.json): |
Proceed ([y]/n)? y
Downloading and Extracting Packages
sqlite-3.37.1 | 1.5 MB | ##################################### | 100%
pip-20.0.2 | 1.9 MB | ##################################### | 100%
zlib-1.2.11 | 88 KB | ##################################### | 100%
ncurses-6.3 | 1012 KB | ##################################### | 100%
readline-8.1 | 295 KB | ##################################### | 100%
python_abi-2.7 | 4 KB | ##################################### | 100%
python-2.7.15 | 12.2 MB | ##################################### | 100%
setuptools-36.4.0 | 557 KB | ##################################### | 100%
libgcc-ng-11.2.0 | 906 KB | ##################################### | 100%
ca-certificates-2021 | 139 KB | ##################################### | 100%
libstdcxx-ng-11.2.0 | 4.2 MB | ########################9 | 68%
…
certifi-2016.9.26 | 217 KB | ##################################### | 100%
Preparing transaction: done
Verifying transaction: done
Executing transaction: done
#
# To activate this environment, use
#
# $ conda activate wes
#
# To deactivate an active environment, use
#
# $ conda deactivate
# conda environments:
#
bioinfo /data1/jiarongf/anaconda3/envs/bioinfo
r-reticulate /data1/jiarongf/anaconda3/envs/r-reticulate
base * /data1/jiarongf/anaconda_se/anaconda3
jupyter_notebook /home/jiarongf/.conda/envs/jupyter_notebook
celltalk /home/jiarongf/my-envs/celltalk
chipseq /home/jiarongf/my-envs/chipseq
d2l /home/jiarongf/my-envs/d2l
pyscenic /home/jiarongf/my-envs/pyscenic
wes /home/jiarongf/my-envs/wes
creat-wes-envs.sh: 12: creat-wes-envs.sh: source: not found
11:22:15 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
source activate wes
(/home/jiarongf/my-envs/wes) 11:23:05 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
prefetch -help
Usage: prefetch [ options ] [ accessions(s)... ]
Parameters:
accessions(s) list of accessions to process
Options:
-T|--type <file-type> Specify file type to download. Default: sra
-N|--min-size <size> Minimum file size to download in KB
(inclusive).
-X|--max-size <size> Maximum file size to download in KB
(exclusive). Default: 20G
-f|--force <no|yes|all|ALL> Force object download - one of: no, yes,
all, ALL. no [default]: skip download if
the object if found and complete; yes:
download it even if it is found and is
complete; all: ignore lock files (stale
locks or it is being downloaded by
another process - use at your own
risk!); ALL: ignore lock files, restart
download from beginning
-p|--progress Show progress
-r|--resume <yes|no> Resume partial downloads - one of: no, yes
[default]
-C|--verify <yes|no> Verify after download - one of: no, yes
[default]
-c|--check-all Double-check all refseqs
-o|--output-file <file> Write file to <file> when downloading
single file
-O|--output-directory <directory>
Save files to <directory>/
--ngc <path> <path> to ngc file
--perm <path> <path> to permission file
--location <location> location in cloud
--cart <path> <path> to cart file
-V|--version Display the version of the program
-v|--verbose Increase the verbosity of the program
status messages. Use multiple times for
more verbosity.
-L|--log-level <level> Logging level as number or enum string.
One of
(fatal|sys|int|err|warn|info|debug) or
(0-6) Current/default is warn
--option-file file Read more options and parameters from the
file.
-h|--help print this message
"prefetch" version 2.11.0
(/home/jiarongf/my-envs/wes) 11:25:16 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
prefetch秧荆，默認通過https下載數(shù)據(jù)倔毙，但是速度不理想。
aspera的下載速度很快乙濒，但是它不是SRA Toolkit的套件之一陕赃，不能用conda安裝，需要下載安裝腳本
wget https://d3gcli72yxqn2z.cloudfront.net/connect_latest/v4/bin/ibm-aspera-connect_4.1.3.93_linux.tar.gz
tar -zxvf ibm-aspera-connect_4.1.3.93_linux.tar.gz
bash ibm-aspera-connect_4.1.3.93_linux.sh
## 需要手動添加環(huán)境變量
export PATH='$HOME/.aspera/connect/bin:$PATH'
source ~/.bashrc
##私鑰文件位于 $HOME/.aspera/connect/etc
## 這是調(diào)用的是aspera
nohup prefetch --option-file ../data/SRR_Acc_List.txt -O ../0.sra -X 200G > ../log/0.download_sra.log 2>&1 &
自己運行
(/home/jiarongf/my-envs/wes) 11:45:36 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
vim download-aspera.sh
(/home/jiarongf/my-envs/wes) 11:52:32 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
cat download-aspera.sh
wget https://d3gcli72yxqn2z.cloudfront.net/connect_latest/v4/bin/ibm-aspera-connect_4.1.3.93_linux.tar.gz
tar -zxvf ibm-aspera-connect_4.1.3.93_linux.tar.gz
bash ibm-aspera-connect_4.1.3.93_linux.sh
(/home/jiarongf/my-envs/wes) 11:50:16 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
sh download-aspera.sh 2> download-aspera.log
ibm-aspera-connect_4.1.3.93_linux.sh
Installing IBM Aspera Connect
Deploying IBM Aspera Connect (/home/jiarongf/.aspera/connect) for the current user only.
Install complete.
(/home/jiarongf/my-envs/wes) 11:51:00 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
vim ~/.bashrc
(/home/jiarongf/my-envs/wes) 11:55:28 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
export PATH=/data1/jiarongf/learning/cancer-WES/run/:$PATH
(/home/jiarongf/my-envs/wes) 11:56:30 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
source ~/.bashrc
11:57:27 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
source activate wes
(/home/jiarongf/my-envs/wes) 11:58:09 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
vim prefetch.sh
(/home/jiarongf/my-envs/wes) 11:59:18 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
cat prefetch.sh
nohup prefetch --option-file ../data/SRR_Acc_List.txt -O ../0.sra -X 200G > ../log/0.download_sra.log 2>&1 &
(/home/jiarongf/my-envs/wes) 11:59:27 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
sh prefetch.sh
(/home/jiarongf/my-envs/wes) 11:59:34 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/run
$
(/home/jiarongf/my-envs/wes) 12:05:06 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/log
$
cat 0.download_sra.log
2022-03-31T04:03:25 prefetch.2.11.0: 1) Downloading 'SRR3182418'...
2022-03-31T04:03:25 prefetch.2.11.0: Downloading via HTTPS...
(/home/jiarongf/my-envs/wes) 12:05:11 jiarongf@172.16.10.223:/data1/jiarongf/learning/cancer-WES/log
$
簡單看一下下載好的log
2022-04-02T00:09:59 prefetch.2.11.0: 49) Downloading 'SRR3182442.vdbcache'...
2022-04-02T00:09:59 prefetch.2.11.0: 49) 'SRR3182442.vdbcache' was downloaded successfully
2022-04-02T00:10:01 prefetch.2.11.0: 50) Downloading 'SRR3182443'...
2022-04-02T00:10:01 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T00:59:08 prefetch.2.11.0: HTTPS download succeed
2022-04-02T00:59:08 prefetch.2.11.0: 50.2) Downloading 'SRR3182443.vdbcache'...
2022-04-02T00:59:08 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T00:59:24 prefetch.2.11.0: HTTPS download succeed
2022-04-02T00:59:24 prefetch.2.11.0: 'SRR3182443.vdbcache' is valid
2022-04-02T00:59:24 prefetch.2.11.0: 50.2) 'SRR3182443.vdbcache' was downloaded successfully
2022-04-02T00:59:36 prefetch.2.11.0: 'SRR3182443' is valid
2022-04-02T00:59:36 prefetch.2.11.0: 50) 'SRR3182443' was downloaded successfully
2022-04-02T01:00:01 prefetch.2.11.0: 'SRR3182443' has 0 unresolved dependencies
2022-04-02T01:00:01 prefetch.2.11.0: 50) Downloading 'SRR3182443.vdbcache'...
2022-04-02T01:00:01 prefetch.2.11.0: 50) 'SRR3182443.vdbcache' was downloaded successfully
2022-04-02T01:00:03 prefetch.2.11.0: 51) Downloading 'SRR3182444'...
2022-04-02T01:00:03 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T02:03:30 prefetch.2.11.0: HTTPS download succeed
2022-04-02T02:03:30 prefetch.2.11.0: 51.2) Downloading 'SRR3182444.vdbcache'...
2022-04-02T02:03:30 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T02:03:47 prefetch.2.11.0: HTTPS download succeed
2022-04-02T02:03:47 prefetch.2.11.0: 'SRR3182444.vdbcache' is valid
2022-04-02T02:03:47 prefetch.2.11.0: 51.2) 'SRR3182444.vdbcache' was downloaded successfully
2022-04-02T02:03:58 prefetch.2.11.0: 'SRR3182444' is valid
2022-04-02T02:03:58 prefetch.2.11.0: 51) 'SRR3182444' was downloaded successfully
2022-04-02T02:04:24 prefetch.2.11.0: 'SRR3182444' has 0 unresolved dependencies
2022-04-02T02:04:24 prefetch.2.11.0: 51) Downloading 'SRR3182444.vdbcache'...
2022-04-02T02:04:24 prefetch.2.11.0: 51) 'SRR3182444.vdbcache' was downloaded successfully
2022-04-02T02:04:26 prefetch.2.11.0: 52) Downloading 'SRR3182445'...
2022-04-02T02:04:26 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T02:56:41 prefetch.2.11.0: HTTPS download succeed
2022-04-02T02:56:41 prefetch.2.11.0: 52.2) Downloading 'SRR3182445.vdbcache'...
2022-04-02T02:56:41 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T02:57:04 prefetch.2.11.0: HTTPS download succeed
2022-04-02T02:57:04 prefetch.2.11.0: 'SRR3182445.vdbcache' is valid
2022-04-02T02:57:04 prefetch.2.11.0: 52.2) 'SRR3182445.vdbcache' was downloaded successfully
2022-04-02T02:57:13 prefetch.2.11.0: 'SRR3182445' is valid
2022-04-02T02:57:13 prefetch.2.11.0: 52) 'SRR3182445' was downloaded successfully
2022-04-02T02:57:37 prefetch.2.11.0: 'SRR3182445' has 0 unresolved dependencies
2022-04-02T02:57:37 prefetch.2.11.0: 52) Downloading 'SRR3182445.vdbcache'...
2022-04-02T02:57:37 prefetch.2.11.0: 52) 'SRR3182445.vdbcache' was downloaded successfully
2022-04-02T02:57:40 prefetch.2.11.0: 53) Downloading 'SRR3182446'...
2022-04-02T02:57:40 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T20:49:37 prefetch.2.11.0: HTTPS download succeed
2022-04-02T20:49:37 prefetch.2.11.0: 53.2) Downloading 'SRR3182446.vdbcache'...
2022-04-02T20:49:37 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T20:49:54 prefetch.2.11.0: HTTPS download succeed
2022-04-02T20:49:54 prefetch.2.11.0: 'SRR3182446.vdbcache' is valid
2022-04-02T20:49:54 prefetch.2.11.0: 53.2) 'SRR3182446.vdbcache' was downloaded successfully
2022-04-02T20:50:06 prefetch.2.11.0: 'SRR3182446' is valid
2022-04-02T20:50:06 prefetch.2.11.0: 53) 'SRR3182446' was downloaded successfully
2022-04-02T20:50:31 prefetch.2.11.0: 'SRR3182446' has 0 unresolved dependencies
2022-04-02T20:50:31 prefetch.2.11.0: 53) Downloading 'SRR3182446.vdbcache'...
2022-04-02T20:50:31 prefetch.2.11.0: 53) 'SRR3182446.vdbcache' was downloaded successfully
2022-04-02T20:50:33 prefetch.2.11.0: 54) Downloading 'SRR3182447'...
2022-04-02T20:50:33 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T21:03:02 prefetch.2.11.0: HTTPS download succeed
2022-04-02T21:03:02 prefetch.2.11.0: 54.2) Downloading 'SRR3182447.vdbcache'...
2022-04-02T21:03:02 prefetch.2.11.0: Downloading via HTTPS...
2022-04-02T21:03:17 prefetch.2.11.0: HTTPS download succeed
2022-04-02T21:03:17 prefetch.2.11.0: 'SRR3182447.vdbcache' is valid
2022-04-02T21:03:17 prefetch.2.11.0: 54.2) 'SRR3182447.vdbcache' was downloaded successfully
2022-04-02T21:03:21 prefetch.2.11.0: 'SRR3182447' is valid
2022-04-02T21:03:21 prefetch.2.11.0: 54) 'SRR3182447' was downloaded successfully
2022-04-02T21:03:46 prefetch.2.11.0: 'SRR3182447' has 0 unresolved dependencies
2022-04-02T21:03:46 prefetch.2.11.0: 54) Downloading 'SRR3182447.vdbcache'...
2022-04-02T21:03:46 prefetch.2.11.0: 54) 'SRR3182447.vdbcache' was downloaded successfully
下了蠻久的
2022-03-31T04:03:25-2022-04-02T21:03:46
下了兩三天颁股，
