轉錄組數據比對奏路，選用了hisat2，比對之后發(fā)現結果不太懂弃舒，于是整理了這篇筆記與大家分享癞埠，歡迎交流。
此處是一個轉錄組數據hisat2比對結果聋呢，bowtie結果也是這個格式苗踪。
17968900 reads; of these: #reads數目
17968900 (100.00%) were paired; of these: #能配對的reads，由于用的是trimmomatic后的paired clean data坝冕，故100%
4434269 (24.68%) aligned concordantly 0 times #不能比對到基因組上
10748474 (59.82%) aligned concordantly exactly 1 time #比對到基因組上徒探，且唯一匹配，即unique map
2786157 (15.51%) aligned concordantly >1 times #比對到基因組上喂窟，但結果>1次测暗，即不唯一匹配
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4434269 pairs aligned concordantly 0 times; of these: #上述不能比對上的reads中
269467 (6.08%) aligned discordantly 1 time #這些雖然比對上了，但是不合理磨澡，例如方向碗啄、R1 R2之間的距離等
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4164802 pairs aligned 0 times concordantly or discordantly; of these: #剩余的不匹配reads中
8329604 mates make up the pairs; of these:
5311882 (63.77%) aligned 0 times
2073153 (24.89%) aligned exactly 1 time #作為單端來比對，這些可以唯一比對
944569 (11.34%) aligned >1 times #這些不唯一比對
85.22% overall alignment rate #結果是唯一比對+不唯一比對+不能比對中可以單端比對的
要看到稳摄，所謂唯一比對稚字，可以直接從這個報告中計算。
referrence:
https://www.cnblogs.com/leezx/p/8540862.html
https://blog.csdn.net/weixin_30935137/article/details/86530092
