第三部分大意:
另一份由科學雜志發(fā)表的論文更直接地用到了RNA。在那篇論文中作者提到了由酶Cas13和另一種可以反向G-A突變效果的酶組成的堿基修正方法。雖然是針對RNA的堿基修正实抡,但是效果上和DNA一樣:通過將一種堿基換成另一種來改變蛋白質的組成和行為。
這些論文雖然各不相同皆辽,但都表明基因工程的工具正在迅速發(fā)展跛梗。尤基是Cas9的各種變體在不斷用來測試CRISPR-Cas9的方法能否被改進。并用另一種叫作Cpf1的酶被嘗試著用來代替Cas9和CRISPR一起實驗翘骂。
這些發(fā)明堿基修正技術的研究人員甚至希望能夠有機會研究出epigenomic修正技術壁熄。epigenomic 能夠影響到一個細胞的類型。雖然目前看來還很遙遠碳竟,但未來卻擁有無限可能草丧。
Another paper which was published in Science involves RNA more directly. The author describes a base editor made from Cas13 and anther enzyme which can reverse the effect of G to A mutation. Although it works on RNA, the effect is the same s DNA, substituing one base for another and thus changing the composition and activity of protein.
Though the papers are different, they demonstrate that the genetic engineering toolkit is developing rapidly. In particular, variants of Cas9 are used to test whether CRISPR-Cas9 approach can be improved. And? another enzyme called Cpf1 are used to substitute Cas9 to test in conjunction with CRISPR.
These researchers of base editing even hope to reach epigenomic editing. epigenomic can influence the type of a cell. Although it seems a distant prospect, but the possibility is limitless.