參考：
以水稻為例教你如何使用BSA方法進(jìn)行遺傳定位（上篇） - 簡(jiǎn)書 (jianshu.com)
- seqkit：序列處理軟件
conda install seqkit
- fastp：數(shù)據(jù)質(zhì)控
conda install fastp
- bwa：數(shù)據(jù)比對(duì)到參考基因組
conda install bwa
- samtools：處理sam/bam文件
mkdir samtools
cd samtools
wget https://github.com/samtools/samtools/releases/download/1.15.1/samtools-1.15.1.tar.bz2
tar xvf samtools-1.15.1.tar.bz2
cd samtools-1.15.1.tar.bz2/
./configure --prefix=/data/kesimin/softwares/samtools/samtools-1.15.1
make
make install
##添加環(huán)境變量
echo 'PATH=/data/xxx/softwares/samtools/samtools-1.15.1/bin:$PATH' >> ~/.bashrc
source ~/.bashrc
- sambamba：標(biāo)記重復(fù)序列
mkdir sambamba
cd sambamba
wget https://github.com/biod/sambamba/releases/download/v0.8.2/sambamba-0.8.2-linux-amd64-static.gz
gunzip sambamba-0.8.2-linux-amd64-static.gz
mv sambamba-0.8.2-linux-amd64-static.gz sambamba
chmod a+x sambamba
echo 'PATH=/data/xxx/softwares/sambamba/sambamba:$PATH' >> ~/.bashrc
source ~/.bashrc
- bcftools：處理vcf/bcf挂捅，變異檢測(cè)
wget -c https://github.com/samtools/bcftools/releases/download/1.15.1/bcftools-1.15.1.tar.bz2
tar xvf bcftools-1.15.1.tar.bz2
cd bcftools-1.15.1/
./configure --prefix=/data/xxx/softwares/bcftools-1.15.1/htslib-1.15.1
make
make install
###添加進(jìn)環(huán)境變量
echo 'PATH=/data/xxx/softwares/bcftools-1.15.1/htslib-1.15.1/bin:$PATH' >> ~/.bashrc
source ~/.bashrc
- R：數(shù)據(jù)分析
