http://mooc.guokr.com/note/17258/
11.1 錯配修復(fù)Introduction to DNA MIsmatch Repair錯配修復(fù)（MMR）
從此開始講DNA修復(fù)车吹。dna錯配有兩種類型：復(fù)制錯誤和DNA損傷
11.2 錯配識別
錯配識別由一種稱為MutS的蛋白質(zhì)介導(dǎo)
11.3MutS Recognition
MutS把錯配的位點識別出來
11.4 Methyl-Directed Nascent Strand Detection
通過甲基化修飾來識別新生鏈副女，大腸桿菌中来氧。因為新生鏈?zhǔn)俏幢患谆逆湣?/p>
甲基化位點為了保護大腸桿菌的DNA使其不在GATC位點被切
11.5MutH Recognition
MutH酶執(zhí)行辨認(rèn)工作仆抵。是一個在GATC位點切割未甲基化鏈的內(nèi)切酶惠拭。從而會切割錯配的新生鏈。
11.6 缺口介導(dǎo)的新生鏈檢測Nick-Directed Nascent Strand Detection
利用了新DNA有缺口的特點。檢測滑行夾是否存在
11.7MutL Regulation in Other Organisms
11.8 Choosing Which Strand to Repair
11.9Methyl-Directed Strand Repair
11.10Nick-Directed Strand Repair
11.10bAdditional Information
12.1Methyl-Directed MMR Assays
12.2Interpreting Methyl-Directed MMR Assay Results
12.3 In Vivo Reversion Assays
12.4In Vitro MMR Assay
12.5In Vitro MMR Incision Assay and Methylation Detection
