下載Cell Ranger file：
conda activate rna-seq
cd ~/wangzh /miniconda3/envs/rna-seq
wget -O cellranger-6.1.1.tar.gz "https://cf.10xgenomics.com/releases/cell-exp/cellranger-6.1.1.tar.gz?Expires=1629482895&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cHM6Ly9jZi4xMHhnZW5vbWljcy5jb20vcmVsZWFzZXMvY2VsbC1leHAvY2VsbHJhbmdlci02LjEuMS50YXIuZ3oiLCJDb25kaXRpb24iOnsiRGF0ZUxlc3NUaGFuIjp7IkFXUzpFcG9jaFRpbWUiOjE2Mjk0ODI4OTV9fX1dfQ__&Signature=cVEzaun4LDFufubZBGLGl0v0whb4e713qybd0VB~UPVOFKQm5dicK5ezTuaBZypDBwm3OcvIbstXiTg5CzOfKEQ4Tsgp8EFdu38KrDmRudHSSvp3thWqaBSAz2fDkoBWE-BjNIhLecjzWVceWyaLZBdpGC8JX-ML8YhT8DhLkQhVNSU3-veq02qlpZfC~BJQN52KExwWW-JquQulRMjV~8~xEUqSgE0JPWSq5N8YdNaZWeauXB7KKXyp-EJSH2ThK0sK71g05KTe8pg4vQeoKja9Rzi1VSFlTw-AWoH5Aj5VhCpp7xqY2Uom2ntU5XvsS3Y9LQJnHX939Bpz4-SUFw__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"
tar -xzvf cellranger-6.1.1.tar.gz
vim ~/.bashrc
$export PATH=/home/zhanglab_wangzh/wangzh/miniconda3/envs/rna-seq/cellranger-6.1.1:$PATH
source ~/.bashrc
cellranger testrun --id=tiny
下載參考文件
cd ~/wangzh/wangzh/RNA-project/reference/10x
wget https://cf.10xgenomics.com/supp/cell-exp/refdata-gex-GRCh38-2020-A.tar.gz
tar -xzvf refdata-gex-GRCh38-2020-A.tar.gz
