fastp 在2018 發(fā)表于bioinformatics 具體文章地址如下:
https://academic.oup.com/bioinformatics/article/34/17/i884/5093234
fastp是一款非常棒的數(shù)據(jù)質(zhì)控的軟件搬男,可以過濾低質(zhì)量的reads合是,去除adapter 捣染,數(shù)據(jù)截取,生成質(zhì)控報告等等鳞青,由于其是C語言撰寫的,速度非掣昕龋快耳高。具體Github路徑如下:
https://github.com/OpenGene/fastp
fastp軟件參數(shù)說明如下:
SE使用實例:
fastp -i BB127_P_1.fq.gz -o BB127_Lib1_Lane1_R1.fastq -A GATCGGAAGAGCACACGTCTGAACTCCAGTCAC -h BB127_filter.html
PE使用實例:
fastp -i reads.1.fq.gz -I reads.2.fq.gz -o clean.1.fq.gz -O clean.2.fq.gz -z 4 -q 20 -u 30 -n 10 -h stat.html
質(zhì)控報告產(chǎn)出如下: